Translational_Unit

Part:BBa_K371000

Designed by: Yang Zhang   Group: iGEM10_USTC   (2010-08-27)

pduJK(Propanediol utilization gene J+K)[RBS+pduJ+RBS+pduK]

Pdu, a abbreviation of propanediol utilization, is name of operon which is used for degradation propanediol to generate energy. Pdu operon consist of 21 genes. Some of these genes encode a series proteins which assemble to a large icosahedral protein complex. PduJK consist of pdu J and pdu K wich their natural RBS, which got from Citrobacter Freundii by PCR. PduJK, together with others struture part of pdu operon, can be used to construct a empty proteinous microcompartment in bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 566
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apduJK(Propanediol utilization gene J+K)[RBS+pduJ+RBS+pduK]
originCitrobacter Freundii